LOCUS       40924_14490        5768 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pDESTR DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Abe A, Elegado EB, Sone T.
  TITLE     Construction of pDESTR, a GATEWAY vector for gene disruption in 
            filamentous fungi
  JOURNAL   Curr. Microbiol. 52 (3), 210-215 (2006)
  PUBMED    16479358
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Abe A, Elegado EB, Sone T.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-2005) Teruo Sone, Graduate School of Agriculture, 
            Hokkaido University, Laboratory of Applied Microbiology; Kita-9 
            Nishi-9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan 
            (E-mail:sonet@chem.agr.hokudai.ac.jp, Tel:81-11-706-2502, 
            Fax:81-11-706-4961)
REFERENCE   3  (bases 1 to 5768)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5768)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr. 
            Microbiol."; date: "2006"; volume: "52"; issue: "3"; pages: 
            "210-215"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-JUN-2005) Teruo Sone, Graduate School of Agriculture, Hokkaido 
            University, Laboratory of Applied Microbiology"; volume: " Kita-9 
            Nishi-9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan 
            (E-mail:sonet@chem.agr.hokudai.ac.jp, Tel:81-11-706-2502, Fax"; 
            pages: "81-11-706-4961"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      26..481
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        559..663
                     /label=AmpR promoter
     CDS             664..1521
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1695..2283
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2564..2918
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             2923..3945
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    4060..4184
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        4209..4239
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             4293..4949
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             5294..5596
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(5640..5764)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt
       61 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
      121 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
      181 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
      241 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
      301 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
      361 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
      421 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
      481 tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atcaggtggc
      541 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      601 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      661 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      721 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      781 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
      841 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
      901 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
      961 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     1021 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1081 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1141 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1201 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1261 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1321 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1381 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1441 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1501 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1561 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1621 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1681 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1741 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1801 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     1861 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     1921 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     1981 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2041 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2101 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2161 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2221 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2281 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2341 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2401 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     2461 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     2521 gaagaacgtt ttccaatgat gagcactttt tgatcgacgt taactgatat tgaaggagca
     2581 ttttttgggc ttggctggag ctagtggagg tcaacaatga atgcctattt tggtttagtc
     2641 gtccaggcgg tgagcacaaa atttgtgtcg tttgacaaga tggttcattt aggcaactgg
     2701 tcagatcagc cccacttgta gcagtagcgg cggcgctcga agtgtgactc ttattagcag
     2761 acaggaacga ggacattatt atcatctgct gcttggtgca cgataacttg gtgcgtttgt
     2821 caagcaaggt aagtggacga cccggtcata ccttcttaag ttcgcccttc ctccctttat
     2881 ttcagattca atctgactta cctattctac ccaagcatcc aaatgaaaaa gcctgaactc
     2941 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     3001 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     3061 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     3121 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggagtt cagcgagagc
     3181 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
     3241 gaactgcccg ctgttctcca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat
     3301 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     3361 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     3421 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     3481 gactgccccg aagtccggca cctcgtgcat gcggatttcg gctccaacaa tgtcctgacg
     3541 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     3601 tacgaggtcg ccaacatcct cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     3661 cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcgcctccg ggcgtatatg
     3721 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     3781 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
     3841 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
     3901 gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagag tagatgccga
     3961 ccgggaacca gttaacgtcg atcagaaaaa aaggatcata tcgtcaatta ttacctccac
     4021 gggctggcgt aatagcgaag aggcccgcac cgatcatcaa caagtttgta caaaaaagct
     4081 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
     4141 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca
     4201 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc
     4261 cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
     4321 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
     4381 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
     4441 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
     4501 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
     4561 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
     4621 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
     4681 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct
     4741 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
     4801 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
     4861 aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
     4921 agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta ctaaaagcca
     4981 gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt
     5041 atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga
     5101 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc
     5161 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag
     5221 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac
     5281 aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
     5341 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct
     5401 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
     5461 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat
     5521 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
     5581 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc
     5641 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
     5701 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
     5761 tggttgat
//
